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Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine®

Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine®

Item no.	BOS-EK2227
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology Neuroscience Neural Adhesion Molecules By Cell Type ELISA Immunostaining
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<75 pg/ml
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	ITGA5
Manufacturer - Specificity	Natural and recombinant human ITGA5
Background	Integrin alpha-5 is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Immunogen	Expression system for standard: NS0; Immunogen sequence: F42-Y995
Description	Human Intergrin Alpha 5/CD49E PicoKine® ELISA Kit (96 Tests). Quantitate Human Intergrin Alpha 5/CD49E in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1, 250 \| 2, 500 \| 5, 000 \| 10, 000 O.D. \| 0.024 \| 0.041 \| 0.084 \| 0.237 \| 0.564 \| 1.033 \| 1.525 \| 2.022
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 242 \| 502 \| 2865 \| 207 \| 579 \| 4142 Standard deviation \| 18.27 \| 39.11 \| 115.46 \| 13.50 \| 32.48 \| 277.93 CV(%) \| 7.6% \| 7.8% \| 4% \| 6.5% \| 5.6% \| 6.7%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 242 \| 166 \| 180 \| 215 \| 201 \| 9.23 \| 4.6% Sample 2 \| 502 \| 527 \| 467 \| 385 \| 470 \| 32.92 \| 7% Sample 3 \| 2865 \| 4075 \| 4582 \| 2563 \| 3521 \| 196.49 \| 5.6% *number of samples for each test n=16.
Capture antibody and Detection antibody	a polyclonal antibody from goat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EK2227-datasheet.pdf

BOS-EK2227-safety-datasheet.pdf