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Human PDGFRB ELISA Kit PicoKine®

Human PDGFRB ELISA Kit PicoKine®

Item no.	BOS-EK2251
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology Neuroscience Neural Lineage Markers By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma ELISA Immunostaining
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<75 pg/ml
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	PDGFRB
Manufacturer - Specificity	Natural and recombinant human PDGFRB
Background	Platelet-derived growth factor receptor beta is a protein that in humans is encoded by the PDGFRB gene. The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
Immunogen	Expression system for standard: NS0; Immunogen sequence: L33-F530
Description	Human PDGFRB PicoKine® ELISA Kit (96 Tests). Quantitate Human PDGFRB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1, 250 \| 2, 500 \| 5, 000 \| 10, 000 O.D. \| 0.033 \| 0.094 \| 0.193 \| 0.402 \| 0.625 \| 0.918 \| 1.334 \| 2.023
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 263 \| 497 \| 3230 \| 268 \| 580 \| 3309 Standard deviation \| 7.18 \| 18.69 \| 69.42 \| 8.58 \| 30.91 \| 218.72 CV(%) \| 2.7% \| 3.8% \| 2% \| 3.2% \| 5.3% \| 6.6%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 263 \| 268 \| 172 \| 274 \| 244 \| 9.09 \| 3.7% Sample 2 \| 497 \| 618 \| 539 \| 507 \| 540 \| 21.5 \| 4% Sample 3 \| 3230 \| 3108 \| 3988 \| 2566 \| 3223 \| 194.35 \| 6% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EK2251-datasheet.pdf

BOS-EK2251-safety-datasheet.pdf