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Human PKLR ELISA Kit PicoKine®

Human PKLR ELISA Kit PicoKine®

Item no.	BOS-EK2257
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Metabolism Glucose Homeostasis ELISA Immunostaining
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<45 pg/ml
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	PKLR
Manufacturer - Specificity	Natural and recombinant human PKLR
Background	Pyruvate kinase PKLR is an enzyme that in humans is encoded by the PKLR gene. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: L47-S574
Description	Human PKLR ELISA Kit PicoKine® (96 Tests). Quantitate Human PKLR in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 45 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sample Data	Concentration (pg/ml) \| 0 \| 93.7 \| 187.5 \| 375 \| 750 \| 1, 500 \| 3, 000 \| 6, 000 O.D. \| 0.031 \| 0.087 \| 0.156 \| 0.365 \| 0.542 \| 0.761 \| 1.413 \| 2.048
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 185 \| 335 \| 2366 \| 185 \| 267 \| 1851 Standard deviation \| 7.25 \| 13.97 \| 57.97 \| 8.94 \| 18.42 \| 116.8 CV(%) \| 3.9% \| 4.2% \| 2.5% \| 4.8% \| 6.9% \| 6.3%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 185 \| 168 \| 186 \| 177 \| 179 \| 13.75 \| 7.7% Sample 2 \| 335 \| 263 \| 278 \| 311 \| 297 \| 11.07 \| 3.7% Sample 3 \| 2366 \| 1875 \| 2286 \| 2162 \| 2172 \| 144.02 \| 6.6% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EK2257-datasheet.pdf

BOS-EK2257-safety-datasheet.pdf