Rat CD25/IL2RA ELISA Kit PicoKine®

PicoKine® ELISA Kits

Il2ra

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Rat CD25/IL2RA ELISA Kit PicoKine® (96 Tests). Quantitate Rat CD25/IL2RA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1, 500 | 3, 000 | 6, 000
O.D. | 0.019 | 0.084 | 0.156 | 0.395 | 0.581 | 0.881 | 1.275 | 1.831

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 114 | 154 | 167 | 139 | 144 | 4.69 | 3.3%
Sample 2 | 306 | 243 | 257 | 203 | 252 | 19.55 | 7.8%
Sample 3 | 2233 | 2818 | 2456 | 2488 | 2499 | 151.67 | 6.1%
*number of samples for each test n=16.

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.