Item no. |
BOS-PB10022-10ug |
Manufacturer |
Boster
|
Amount |
10 ug |
Format |
Lyophilized |
Applications |
WB, FC, IF, IHC, ICC, IHC-F |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Rabbit |
Isotype |
IgG |
Sensitivity |
>5000 cells |
Citations |
1. Cao W, Liu N, Tang S, Bao L, Shen L, Yuan H, Zhao X, Lu H (Jun 2008). "Acetyl-Coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines". Biochimica et Biophysica Acta 1780 (6): 873–80. 2. Kathiresan S, Melander O, Guiducci C, Surti A, Burtt NP, Rieder MJ, Cooper GM, Roos C, Voight BF, Havulinna AS, Wahlstrand B, Hedner T, Corella D, Tai ES, Ordovas JM, Berglund G, Vartiainen E, Jousilahti P, Hedblad B, Taskinen MR, Newton-Cheh C, Salomaa V, Peltonen L, Groop L, Altshuler DM, Orho-Melander M (Feb 2008). "Six new loci associated with blood low-absorbance lipoprotein cholesterol, high-absorbance lipoprotein cholesterol or triglycerides in humans". Nature Genetics 40 (2): 189–97. |
Alias |
3-ketoacyl-CoA thiolase, mitochondrial;2.3.1.16;Acetyl-CoA acyltransferase;Beta-ketothiolase;Mitochondrial 3-oxoacyl-CoA thiolase;T1;ACAA2; |
Available |
|
Manufacturer - Category |
Primary Antibodies |
Manufacturer - Isotype |
Rabbit IgG |
Storage Conditions |
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Observed Molecular Weight |
42 kDa |
Calculated Molecular weight |
41924 MW |
Clonality |
Polyclonal |
Application Details |
Western blot, 0.1-0.5?g/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1?g/ml, Human
Immunocytochemistry/Immunofluorescence, 2?g/ml, Human
Flow Cytometry (Fixed), 1-3?g/1x106 cells, Human |
Manufacturer - Gene Name |
ACAA2 |
Gene Full Name |
3-ketoacyl-CoA thiolase, mitochondrial |
Manufacturer - Specificity |
No cross reactivity with other proteins. |
Background |
3-Ketoacyl-CoA thiolase, mitochondrial, also known as acetyl-Coenzyme A acyltransferase 2, is an acetyl-CoA C-acyltransferase enzyme that in humans is encoded by the ACAA2 gene. The ACAA2 gene encodes a 41.9 kDa protein that is composed of 397 amino acids and contains 88 observed peptides. The encoded protein catalyzes the last step of themitochondrial fatty acid beta oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. Additionally, ACAA2 has been shown to be a functional BNIP3 binding partner, which provides a possible link between fatty acid metabolism and cell apoptosis. |
Immunogen |
A synthetic peptide corresponding to a sequence in the middle region of human ACAA2, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
Contents |
Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3. |
Purification |
Immunogen affinity purified. |
Reconstitution |
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml. |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. |
Manufacturer - Research Category |
Cardiovascular, Cholesterol Metabolism, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Pathways and Processes, Redox Metabolism, Signal Transduction, Subcellular Markers, Tags & Cell Markers |
Protein Name |
3-ketoacyl-CoA thiolase, mitochondrial |
Protein Function |
Abolishes BNIP3-mediated apoptosis and mitochondrial damage. |
Subcellular Localization |
Mitochondrion. Colocalizes with BNIP3 in the mitochondria. |
Description |
Boster Bio Anti-ACAA2 Antibody Picoband® catalog # PB10022. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. |
Tissue Specificity |
Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level). |
Sequence Similarities |
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily. |