« Back
Home /
Anti-PU.1/Spi1 Antibody

Anti-PU.1/Spi1 Antibody

Item no.	BOS-RP1097-HRP
Manufacturer	Boster
Amount	100 ug
Format	Lyophilized
Applications	IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	HRP
Sensitivity	<1pg/ml
Citations	1. "Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1". 2. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998). "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS". J. Biol. Chem. 273 (9): 4838–42. 3. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990). "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors". Oncogene 5 (5): 663–8.
Alias	Transcription factor PU.1;31 kDa-transforming protein;SPI1;
Available
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	150 kDa
Calculated Molecular weight	31083 MW
Clonality	Polyclonal
Application Details	Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Human, Mouse, Rat, By Heat
Manufacturer - Gene Name	SPI1
Gene Full Name	Transcription factor PU.1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen	E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196). Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.
Contents	Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Adaptive Immunity, B Cells, DNA/RNA, Domain Families, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Non-CD, RNA Processing, Splicing, Transcription, Transcription Factors
Protein Name	Transcription factor PU.1
Protein Function	Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Subcellular Localization	Nucleus.
Description	Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 100 ug

available

Delivery expected until 6/12/2025

Express Delivery: Save 3 days

Compare

Add to wishlist

Recommend by mail