Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband®

Primary Antibodies

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

115281 MW

Western blot, 0.1-0.5?g/ml, Human, Mouse, Rat

Immunocytochemistry/Immunofluorescence, 2?g/ml, Human

Protein phosphatase 1 regulatory subunit 12A

PPP1R12A (Protein phosphatase 1 regulatory subunit 12A), also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1, 030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.

Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Motor Proteins, Myosin, Signal Transduction

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.

Boster Bio Anti-Myosin Phosphatase/PPP1R12A Antibody catalog # PA1681. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Contains 6 ANK repeats.