Comparison

Anti-Myeloperoxidase MPO Antibody European Partner

Item no. BOS-A00372
Manufacturer Boster
Amount 2 ml
Category
Type Antibody
Format Lyophilized
Applications WB
Specific against Human (Homo sapiens)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Myeloperoxidase, Myeloperoxidase, MPO
Available
Manufacturer - Category
Primary Antibodies
Manufacturer - Isotype
Antiserum
Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Clonality
Polyclonal
Application notes
Anti-myeloperoxidase antibody has been tested in western blotting. This antibody is suitable for ELISA and immunoprecipitation. The antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combination (68 kDa and 106 kDa). Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. Anti-Human Myeloperoxidase may react with MPO from other sources. Anti-Human Myeloperoxidase detects neutrophilic granulocytes and monocytes in blood and precursors of granulocytes in the bone marrow. This antibody may also detect myeloid leukemias of the bone marrow as well as other sites.
Application Details
ELISA: 1:200, 000
IP: 1:100
WB: 1:5, 000
Manufacturer - Gene Name
MPO
Background
Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114, 000 to 140, 000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
Immunogen
Myeloperoxidase [Human Leukocytes]
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Purification
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]. Cross-reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL
Concentration
75 mg/mL by Refractometry
Manufacturer - Research Category
Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Pathways and Processes, Redox Metabolism, Vasculature
Short Description
Boster Bio Anti-Myeloperoxidase MPO Antibody (Catalog # A00372). Tested in WB applications. This antibody reacts with Human.
Description
Boster Bio Anti-Myeloperoxidase MPO Antibody (Catalog # A00372). Tested in WB applications. This antibody reacts with Human.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 2 ml
Available: In stock
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