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Anti-Collagen II/COL2A1 Antibody Picoband®

Anti-Collagen II/COL2A1 Antibody Picoband®

Item no.	BOS-A00517-1
Manufacturer	Boster
Amount	100 ug/vial
Category	Antibodies Cell Biology Cell Movement Cell Adhesion Western Blot Cell Stainings ECM Primary Antibodies
Type	Antibody Primary
Format	Lyophilized
Applications	WB
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Citations	1. Donahue, L. R., Chang, B., Mohan, S., Miyakoshi, N., Wergedal, J. E., Baylink, D. J., Hawes, N. L., Rosen, C. J., Ward-Bailey, P., Zheng, Q. Y., Bronson, R. T., Johnson, K. R., Davisson, M. T. A missense mutation in the mouse Col2a1 gene causes spondyloepiphyseal dysplasia congenita, hearing loss, and retinoschisis. J. Bone Miner. Res. 18: 1612-1621, 2003. Note: Erratum: J. Bone Miner. Res. 22: 2011 only, 2007. 2. Faivre, L., Le Merrer, M., Douvier, S., Laurent, N., Thauvin-Robinet, C., Rousseau, T., Vereecke, I., Sagot, P., Delezoide, A.-L., Coucke, P., Mortier, G. Recurrence of achondrogenesis type II within the same family: evidence for germline mosaicism. Am. J. Med. Genet. 126A: 308-312, 2004.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Collagen alpha-1 (II) chain; Alpha-1 type II collagen; Collagen alpha-1 (II) chain; Chondrocalcin
Available
Specificity	No cross reactivity with other proteins.
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	142 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5?g/ml
Manufacturer - Gene Name	COL2A1
Gene Full Name	collagen, type II, alpha 1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, which shares 91.7% and 95.8% amino acid (aa) sequence identity with mouse and rat Collagen II, respectively.
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures
Protein Function	Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Subcellular Localization	Secreted, extracellular space, extracellular matrix.
Description	Boster Bio Anti-Collagen II/COL2A1 Antibody Picoband® catalog # A00517-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A00517-1-datasheet.pdf

BOS-A00517-1-safety-datasheet.pdf