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Anti-DIL-2 TPX2 Antibody

Anti-DIL-2 TPX2 Antibody

Item no.	BOS-A01610
Manufacturer	Boster
Amount	100 ul
Category	Antibodies Cell Biology Cell Culture Cytokines Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Targeting protein for Xklp2;Differentially expressed in cancerous and non-cancerous lung cells 2;DIL-2;Hepatocellular carcinoma-associated antigen 519;Hepatocellular carcinoma-associated antigen 90;Protein fls353;Restricted expression proliferation-associated protein 100;p100;TPX2;C20orf1, C20orf2, DIL2, HCA519;
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	85653 MW
Clonality	Polyclonal
Application Details	Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100
Manufacturer - Gene Name	TPX2
Gene Full Name	Targeting protein for Xklp2
Background	Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. Manda R., Genomics 61:5-14(1999). Zhang Y., Cytogenet. Cell Genet. 84:182-183(1999). Wang Y., J. Immunol. 169:1102-1109(2002).
Immunogen	Synthesized peptide derived from internal of human DIL-2.
Contents	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Cell Division, Chromosome Structure, Epigenetics and Nuclear Signaling, Spindle
Protein Function	Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).
Subcellular Localization	Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
Short Description	Boster Bio Anti-DIL-2 TPX2 Antibody (Catalog # A01610). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Description	Boster Bio Anti-DIL-2 TPX2 Antibody (Catalog # A01610). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Tissue Specificity	Expressed in lung carcinoma cell lines but not in normal lung tissues.

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BOS-A01610-datasheet.pdf

BOS-A01610-safety-datasheet.pdf