Anti-Histone H4 HIST1H4A Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Liquid (sterile filtered)

Anti-Histone H4 pan Antibody is tested for Western Blot and Dot Blots. This antibody is suitable for Chromatin Immunoprecipitation and ELISA. Expect a single band approximately 11.3 kDa in size corresponding to H4 by western blot in the appropriate tissue or cell lysate. Specific conditions for reactivity should be optimized by the end user.

HIST1H4A

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

90 mg/mL by Refractometry

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Boster Bio Anti-Histone H4 HIST1H4A Antibody (Catalog # A14495). Tested in Dot blot, WB applications. This antibody reacts with Human.