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Anti-Ski8 WDR61 Antibody

Anti-Ski8 WDR61 Antibody

Item no.	BOS-CI1032
Manufacturer	Boster
Amount	150 ug/60 ul
Category	Antibodies Immunoprecipitation Western Blot Primary Antibodies CHiP
Type	Antibody
Format	Liquid
Applications	WB, CHIP
Specific against	Human (Homo sapiens), Drosophila
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Molecular Weight	33581 MW
Clonality	Polyclonal
Gene Name	WDR61
Gene Full Name	WD repeat-containing protein 61
Background	SKI8 is a component of the PAF and SKI complex which is composed of SKI2, SKI3 and SKI8. These are involved in transcriptional regulation and events downstream of RNA synthesis. Interaction of the SKI complex with SKI7 links the SKI complex to the exosome in order to perform mRNA degradation.
Contents	Protein G purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Purification	Protein G purified
Concentration	0.5-1 mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Manufacturer - Research Category	Cell Biology, Cell Cycle, Cell Division
Protein Name	WD repeat-containing protein 61
Protein Function	Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly (A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
Description	Boster Bio Anti-Ski8 WDR61 Antibody (Catalog# CI1032). Tested in WB, ChIP applications. This antibody reacts with Drosophila, Human.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-CI1032-datasheet.pdf

BOS-CI1032-safety-datasheet.pdf