Anti-human CD45 PTPRC Monoclonal Antibody Biotin Conjugated, Flow Validated

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Monoclonal

PTPRC

CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

PBS pH 7.2, 0.09% sodium azide

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Receptor-type tyrosine-protein phosphatase C

Membrane; Single-pass type I membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts.

Boster Bio Anti-human CD45 PTPRC Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC00555-Biotin). Tested in Flow Cytometry application(s). This antibody reacts with Human.