Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

Rabbit IgG

36053 MW

Western Blot (WB): 1:1000 dilution

Immunoprecipitation (IP): 1:200 dilution

Chromatin IP (ChIP): 1:200 dilution

Immunocytochemistry (ICC): 1:200 dilution

Immunohistochemistry (IHC): 1:200 dilution.

Glyceraldehyde-3-phosphate dehydrogenase

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (Catalog # M00227-5). Tested in WB, IP, ChIP, ICC, IHC applications. This antibody reacts with Human, Monkey.

Mouse, Goat