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Anti-Rad18 Rabbit Monoclonal Antibody

Anti-Rad18 Rabbit Monoclonal Antibody

Item no.	BOS-M01622
Manufacturer	Boster
Amount	100 ul
Category	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Aging & Neurological Disorders Antibodies Flow Cytometry Western Blot Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC
Clone	FFC-18
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<10pg/ml
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	E3 ubiquitin-protein ligase RAD18;6.3.2.-;Postreplication repair protein RAD18;hHR18;hRAD18;RING finger protein 73;RAD18;RNF73;
Available
Manufacturer - Category	Rabbit Monoclonal Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	28 kDa
Calculated Molecular weight	56223 MW
Clonality	Monoclonal
Application Details	WB 1:10000-1:20000 FC 1:20
Manufacturer - Gene Name	RAD18
Gene Full Name	E3 ubiquitin-protein ligase RAD18
Immunogen	A synthesized peptide derived from human Rad18
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Purification	Affinity-chromatography
Reconstitution	Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Concentration	Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Manufacturer - Research Category	Cell Biology, 2339, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Ubiquitin E3 Enzymes
Protein Name	Lysosome-associated membrane glycoprotein 2 (Lamp2)
Protein Function	E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Subcellular Localization	Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).
Description	Boster Bio Anti-Rad18 Rabbit Monoclonal Antibody catalog # M01622. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Expressed ubiquitously in normal tissues.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M01622-datasheet.pdf

BOS-M01622-safety-datasheet.pdf