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Anti-CD2AP Antibody Picoband® (monoclonal, 5F8)

Anti-CD2AP Antibody Picoband® (monoclonal, 5F8)

Item no.	BOS-M01756
Manufacturer	Boster
Amount	100 ug/vial
Category	Neuroscience Neural Lineage Markers Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC
Clone	5F8
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG1
Sensitivity	<10pg/ml
Citations	1. Cochran, J. N., Rush, T., Buckingham, S. C., Roberson, E. D. The Alzheimer's disease risk factor CD2AP maintains blood-brain barrier integrity. Hum. Molec. Genet. 24: 6667-6674, 2015. 2. Lowik, M. M., Groenen, P. J. T. A., Pronk, I., Lilien, M. R., Goldschmeding, R., Dijkman, H. B., Levtchenko, E. N., Monnens, L. A., van den Heuvel, L. P. Focal segmental glomerulosclerosis in a patient homozygous for a CD2AP mutation. Kidney Int. 72: 1198-1203, 2007.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains; CD2AP
Available
Specificity	No cross reactivity with other proteins.
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Mouse IgG1
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	80 kDa
Clonality	Monoclonal
Application Details	Western blot, 0.1-0.5?g/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml Immunocytochemistry/Immunofluorescence, 5 ?g/ml Flow Cytometry (Fixed), 1-3?g/1x10⁶ cells
Manufacturer - Gene Name	CD2AP
Gene Full Name	CD2 associated protein
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein ly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
Immunogen	E. coli-derived human CD2AP recombinant protein (Position: K253-K337).
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Organelle Proteins, Protein Trafficking, Signal Transduction
Protein Name	activating transcription factor 1
Protein Function	Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T- cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
Subcellular Localization	Cytoplasm, cytoskeleton. Cell projection, ruffle. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.
Description	Boster Bio Anti-CD2AP Antibody Picoband® (monoclonal, 5F8) catalog # M01756. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Widely expressed in fetal and adult tissues.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M01756-datasheet.pdf

BOS-M01756-safety-datasheet.pdf