« Back
Home /
Anti-Nck NCK1 Monoclonal Antibody

Anti-Nck NCK1 Monoclonal Antibody

Item no.	BOS-M02260
Manufacturer	Boster
Amount	100 ul
Category	Antibodies Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IP, IHC, ICC
Clone	AFEG-14
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	>5000 cells
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Oligodendrocyte transcription factor 2;Oligo2;Class B basic helix-loop-helix protein 1;bHLHb1;Class E basic helix-loop-helix protein 19;bHLHe19;Protein kinase C-binding protein 2;Protein kinase C-binding protein RACK17;OLIG2;BHLHB1, BHLHE19, PRKCBP2, RACK17;
Available
Manufacturer - Category	Rabbit Monoclonal Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	16 kDa
Calculated Molecular weight	32385 MW
Clonality	Monoclonal
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 FC 1:500
Manufacturer - Gene Name	NCK1
Gene Full Name	Oligodendrocyte transcription factor 2
Immunogen	A synthesized peptide derived from human Nck Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Purification	Affinity-chromatography
Reconstitution	Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Concentration	Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Manufacturer - Research Category	Actin Assembly, Actin, etc., Adapters, Cancer, Cancer Susceptibility, Cytoplasmic, Cytoskeleton, Cytoskeleton/ECM, Epigenetics and Nuclear Signaling, Microfilaments, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Signal Transduction, Transcription
Protein Name	Lysosome-associated membrane glycoprotein 2 (Lamp2)
Protein Function	Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity).
Subcellular Localization	Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
Description	Boster Bio Anti-Nck NCK1 Monoclonal Antibody catalog # M02260. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M02260-datasheet.pdf

BOS-M02260-safety-datasheet.pdf