« Back
Home /
Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody

Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody

Item no.	BOS-M02305
Manufacturer	Boster
Amount	100 ug
Category	Neuroscience Neural Lineage Markers Antibodies Cell Biology Cytoskeleton Immunohistochemistry Immunofluorescence Primary Antibodies Intermediate filaments
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Clone	SPM623
Specific against	Human (Homo sapiens), Dog (Canine, Canis lupus familiaris), Cat (Feline, Felis catus)
Host	Mouse
Isotype	IgG1 Kappa
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Keratin, type I cytoskeletal 10;Cytokeratin-10;CK-10;Keratin-10;K10;KRT10;KPP;
Available
Manufacturer - Category	Primary Antibodies, Monoclonal Antibodies, 2279
Storage Conditions	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Observed Molecular Weight	58827 MW
Clonality	Monoclonal
Application Details	Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Manufacturer - Gene Name	KRT10
Gene Full Name	Keratin, type I cytoskeletal 10
Background	This monoclonal antibody recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Immunogen	Cytoskeletal preparation extracted from human ectocervical epithelium
Contents	Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purification	200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.
Concentration	Purified antibody with BSA and azide at 200ug/ml
Manufacturer - Research Category	Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Signal Transduction
Subcellular Localization	Cytoplasmic
Short Description	Boster Bio Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody (Catalog # M02305). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Dog, Cat.
Description	Boster Bio Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody (Catalog # M02305). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Dog, Cat.
Tissue Specificity	Seen in all suprabasal cell layers including stratum corneum.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M02305-datasheet.pdf

BOS-M02305-safety-datasheet.pdf