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Anti-Atg4A Monoclonal Antibody

Anti-Atg4A Monoclonal Antibody

Item no.	BOS-M06539
Manufacturer	Boster
Amount	100 ul
Category	Antibodies Cell Biology Cell Status Immunohistochemistry Immunoprecipitation Western Blot Autophagy Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, IP, IHC
Clone	ACHB-1
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<15pg/ml
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Death domain-containing protein CRADD;Caspase and RIP adapter with death domain;RIP-associated protein with a death domain;CRADD;RAIDD;
Available
Manufacturer - Category	Rabbit Monoclonal Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	45 kDa
Calculated Molecular weight	22745 MW
Clonality	Monoclonal
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 IP 1:50
Manufacturer - Gene Name	ATG4A
Gene Full Name	Death domain-containing protein CRADD
Immunogen	A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Purification	Affinity-chromatography
Reconstitution	Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Concentration	Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Manufacturer - Research Category	Cancer, Cardiovascular, Cell Biology, Cell Death, Extracellular Matrix, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
Protein Name	T-cell surface glycoprotein CD3 zeta chain
Protein Function	Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
Subcellular Localization	Cytoplasm. Nucleus.
Description	Boster Bio Anti-Atg4A Monoclonal Antibody catalog # M06539. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M06539-datasheet.pdf

BOS-M06539-safety-datasheet.pdf