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Anti-Phospho-p62 (Ser28) SQSTM1 Antibody

Anti-Phospho-p62 (Ser28) SQSTM1 Antibody

Item no.	BOS-P00300-1
Manufacturer	Boster
Amount	100 ul
Category	Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Signaling Protein Aggregation, Clearance & Misfolding Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	A170 antibody, DMRV antibody, EBI 3 associated protein of 60 kDa antibody, EBI 3 associated protein p60 antibody, EBI3 associated protein of 60 kDa antibody, EBI3 associated protein p60 antibody, EBI3-associated protein of 60 kDa antibody, EBIAP antibody, FTDALS3 antibody, MGC127197 antibody, ORCA antibody, OSF-6 antibody, Osi antibody, OSIL antibody, Oxidative stress induced like antibody, p60 antibody, p62 antibody, p62B antibody, Paget disease of bone 3 antibody, PDB 3 antibody, PDB3 antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody, PKC-zeta-interacting protein antibody, Protein kinase C-zeta-interacting protein antibody, Sequestosome 1 antibody, Sequestosome-1 antibody, SQSTM 1 antibody, SQSTM_HUMAN antibody, Sqstm1 antibody, STAP antibody, STONE14 antibody, Ubiquitin binding protein p62 antibody, Ubiquitin-binding protein p62 antibody, ZIP 3 antibody, ZIP antibody, ZIP3 antibody
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Observed Molecular Weight	47687 MW
Clonality	Polyclonal
Application notes	Specific for endogenous levels of the ~48 kDa p62 protein phosphorylated at Ser28. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Application Details	WB: 1:1000
Manufacturer - Gene Name	SQSTM1
Gene Full Name	Sequestosome-1
Background	p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Nogalaska et al., 2009). p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages (Nakaso et al., 2004). Phosphorylation of Ser-28 has recently been demonstrated to be related to the pathogenesis of Parkinson’s disease.
Immunogen	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser28 of human p62, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
Contents	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Purification	Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Concentration	0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Phospho Site	Phosphorylated, Ser28
Manufacturer - Research Category	Cancer, Cardiovascular, Cell Death, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, NFKB Pathway, Nuclear Signaling, Pathways and Processes, Pol II Transcription, Polymerase Associated Factors, Protein Trafficking, Signal Transduction, Signaling Pathway, Transcription, Vesicle Transport
Protein Name	Sequestosome-1
Protein Function	Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin- 1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K (+) channels.
Subcellular Localization	Cytoplasm. Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Nucleus. Endoplasmic reticulum. Cytoplasm, P-body. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Commonly found in inclusion bodies containing polyubiquitinated protein aggregates. In neurodegenerative diseases, detected in Lewy bodies in Parkinson disease, neurofibrillary tangles in Alzheimer disease, and HTT aggregates in Huntington disease. In protein aggregate diseases of the liver, found in large amounts in Mallory bodies of alcoholic and nonalcoholic steatohepatitis, hyaline bodies in hepatocellular carcinoma, and in SERPINA1 aggregates. Enriched in Rosenthal fibers of pilocytic astrocytoma. In the cytoplasm, observed in both membrane-free ubiquitin- containing protein aggregates (sequestosomes) and membrane- surrounded autophagosomes. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum. Co-localizes with TRIM5 in the cytoplasmic bodies.
Short Description	Boster Bio Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (Catalog # P00300-1). Tested in WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (Catalog # P00300-1). Tested in WB applications. This antibody reacts with Human.
Tissue Specificity	Ubiquitously expressed.
Predicted Reactivity	Primate

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BOS-P00300-1-datasheet.pdf

BOS-P00300-1-safety-datasheet.pdf