Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody

Primary Antibodies

70325 MW

Specific for endogenous levels of the ~76 kDa SERT protein phosphorylated at Thr276. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

SLC6A4

The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at Thr-276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at Thr-276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the Thr-276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Neurotransmitter, Pathways and Processes, Receptors / Channels, Signal Transduction, Transporters

Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

Boster Bio Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody (Catalog # P00496). Tested in WB applications. This antibody reacts with Mouse, Rat.

Expressed in platelets (at protein level).