Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody

Primary Antibodies

77983 MW

Specific for endogenous levels of the ~80 kD 5-LO phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (?-phosphatase, 1200 units for 30 min).

ALOX5

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Endocrine Metabolism, Energy Metabolism, Energy Transfer Pathways, Heart Disease, Hormone Biosynthesis, Lipid and Lipoprotein Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Boster Bio Anti-Phospho-5-Lipoxygenase (Ser523) ALOX5 Antibody (Catalog # P00847). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Primate