Anti-Phospho-Tryptophan Hydroxylase 2 (Ser19) TPH2 Antibody

Primary Antibodies

56057 MW

Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19 .

TPH2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Neuroscience, Neurotransmitter

Boster Bio Anti-Phospho-Tryptophan Hydroxylase 2 (Ser19) TPH2 Antibody (Catalog # P01643). Tested in WB applications. This antibody reacts with Mouse, Rat.

Brain specific.