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Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody

Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody

Item no.	BOS-P02536
Manufacturer	Boster
Amount	100 ul
Category	Neuroscience Neural Signaling Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB, ICC
Specific against	Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Sheep
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Observed Molecular Weight	97295 MW
Clonality	Polyclonal
Application notes	Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by ?-phosphatase treatment.
Application Details	WB: 1:1000 ICC: 1:1000
Manufacturer - Gene Name	DNM1
Gene Full Name	Dynamin-1
Background	Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser-774 and Ser-778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser-774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010).
Immunogen	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
Contents	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Purification	Prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Concentration	0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Phospho Site	Phosphorylated, Ser774
Manufacturer - Research Category	Cell Type Marker, Neuron Marker, Neuroscience, Protein Trafficking, Signal Transduction, Synapse Marker, Vesicle Transport
Protein Name	Dynamin-1
Protein Function	Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis (By similarity).
Subcellular Localization	Cytoplasm. Cytoplasm, cytoskeleton. Microtubule-associated (PubMed:9725914). Isoform-specific localization (PubMed:9725914).
Short Description	Boster Bio Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody (Catalog # P02536). Tested in WB, ICC applications. This antibody reacts with Mouse, Rat.
Description	Boster Bio Anti-Phospho-Dynamin (Ser774) Dnm1 Antibody (Catalog # P02536). Tested in WB, ICC applications. This antibody reacts with Mouse, Rat.
Tissue Specificity	Brain-specific (peripheral sensory neurons).
Predicted Reactivity	Bovine, Canine, Chicken, Human, Primate

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Request Data Sheet

BOS-P02536-datasheet.pdf

BOS-P02536-safety-datasheet.pdf