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Bovine BMP-4 ELISA Kit PicoKine®

Bovine BMP-4 ELISA Kit PicoKine®

Item no.

BOS-EK0314-BV

Manufacturer

Boster

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Cattle (Bovine)

Sensitivity

<4 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Bone morphogenetic protein 4;BMP-4;Bone morphogenetic protein 2B;BMP-2B;BMP4;BMP2B, DVR4;

Available

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

46555 MW

Manufacturer - Gene Name

BMP4

Gene Full Name

Bone morphogenetic protein 4

Sample Type

cell culture supernatants, cell lysates and serum.

Sample Data

Concentration (pg/ml)

Intra Assay Consistency & Inter Assay Consistency

	Intra-Assay Precision			Inter-Assay Precision
Sample
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Background	Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In Bovine embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.
Immunogen	Expression system for standard: NS0; Immunogen sequence: S293-R408
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Nervous System Development, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
Protein Name	Bone morphogenetic protein 4
Protein Function	Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Subcellular Localization	Secreted, extracellular space, extracellular matrix.
Description	Bovine BMP-4 ELISA Kit PicoKine® (96 Tests). Quantitate Bovine BMP4 in cell culture supernatants, cell lysates and serum. Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
Sequence Similarities	Belongs to the TGF-beta family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK0314-BV.pdf

BOS-EK0314-BV-datasheet.pdf

BOS-EK0314-BV-safety-datasheet.pdf