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Human IL-20/Interleukin-20 ELISA Kit PicoKine®

Human IL-20/Interleukin-20 ELISA Kit PicoKine®

Item no.	BOS-EK0599
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Neural Transcription Factors Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Breast Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
Sequence	Belongs to the IL-10 family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-20,IL-20,Cytokine Zcyto10,IL20,ZCYTO10,UNQ852/PRO1801
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	20072 MW
Gene Name	IL20
Gene Full Name	Interleukin-20
Manufacturer - Specificity	Natural and recombinant human IL-20
Background	Interleukin-20 (IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: L25-E176
Manufacturer - Research Category	Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins
Protein Name	Interleukin-20
Protein Function	Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3.
Subcellular Localization	Secreted.
Description	Human IL-20 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the IL-10 family.
Sample Data	Concentration (pg/ml) \| 0 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 \| 4000 O.D. \| 0.090 \| 0.141 \| 0.171 \| 0.285 \| 0.491 \| 0.827 \| 1.546 \| 1.903
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 97 \| 693 \| 1747 \| 90 \| 624 \| 1759 Standard deviation \| 5.72 \| 46.12 \| 132.77 \| 6.12 \| 44.92 \| 138.96 CV (%) \| 7.6% \| 6.9% \| 7.6% \| 6.8% \| 7.2% \| 7.9%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 97 \| 97 \| 98 \| 99 \| 97 \| 0.82 \| 0.8% Sample 2 \| 693 \| 639 \| 709 \| 709 \| 687 \| 28.75 \| 4.1% Sample 3 \| 1747 \| 1963 \| 1758 \| 1970 \| 1859 \| 107.09 \| 5.7% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK0599.pdf

BOS-EK0599-datasheet.pdf

BOS-EK0599-safety-datasheet.pdf