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Human Syndecan-4/SDC4 ELISA Kit PicoKine®

Human Syndecan-4/SDC4 ELISA Kit PicoKine®

Item no.

BOS-EK1356

Manufacturer

Boster

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Syndecan-4;SYND4;Amphiglycan;Ryudocan core protein;SDC4;

Available

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

21642 MW

Manufacturer - Gene Name

SDC4

Gene Full Name

Syndecan-4

Sample Type

cell culture supernatants, cell lysates and serum.

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant human Syndecan-4

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Background	Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4, 5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
Immunogen	Expression system for standard: NS0; Immunogen sequence: E19-E145
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Neurogenesis, Neurology Process, Neuroscience, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers
Protein Name	Syndecan-4
Protein Function	Cell surface proteoglycan that bears heparan sulfate.
Subcellular Localization	Isoform 1: Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.
Description	Human Syndecan-4/SDC4 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC4 in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed in epithelial and fibroblastic cells.

Human Syndecan-4/SDC4 ELISA Kit PicoKine®

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK1356.pdf

BOS-EK1356-datasheet.pdf

BOS-EK1356-safety-datasheet.pdf