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Human B2M / beta 2 Microglobulin ELISA Kit PicoKine®

Human B2M / beta 2 Microglobulin ELISA Kit PicoKine®

Item no.	BOS-EK1691
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology Food Safety Allergenes By Cell Type Hematology Myeloma Melanoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Heart & Blood Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Beta-2-microglobulin,Beta-2-microglobulin form pI 5.3,B2M,CDABP0092,HDCMA22P
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	B2M
Gene Full Name	beta-2-microglobulin
Manufacturer - Specificity	Natural and recombinant human B2M
Background	Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Immunogen	Target protein isolated from human plasma
Manufacturer - Research Category	Cancer, Cardiovascular, Serum Proteins, Tumor Biomarkers
Protein Function	Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
Subcellular Localization	Secreted.
Description	Human B2M / beta 2 Microglobulin ELISA Kit PicoKine® (96 Tests). Quantitate Human B2M in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.049 \| 0.113 \| 0.184 \| 0.225 \| 0.523 \| 0.766 \| 1.328 \| 2.053
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 1103 \| 1070 \| 5097 \| 310 \| 1103 \| 4757 Standard deviation \| 21.97 \| 62.06 \| 219.17 \| 26.97 \| 69.48 \| 285.42 CV (%) \| 7.5% \| 5.8% \| 4.3% \| 8.7% \| 6.3% \| 6%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 293 \| 284 \| 309 \| 337 \| 305 \| 20.14 \| 6.6% Sample 2 \| 1070 \| 1104 \| 1006 \| 1045 \| 1056 \| 35.77 \| 3.3% Sample 3 \| 5097 \| 4645 \| 4569 \| 4545 \| 4714 \| 224.18 \| 4.7% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from mouse\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK1691.pdf

BOS-EK1691-datasheet.pdf

BOS-EK1691-safety-datasheet.pdf