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Human CCL18/PARC ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human CCL18/PARC ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Item no.	BOS-EZ0686
Manufacturer	Boster
Amount	5 plates/kit
Category	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Immunostaining Bone Cancer
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
Sequence	Belongs to the intercrine beta (chemokine CC) family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	C-C motif chemokine 18,Alternative macrophage activation-associated CC chemokine 1,AMAC-1,CC chemokine PARC,Dendritic cell chemokine 1,DC-CK1,Macrophage inflammatory protein 4,MIP-4,Pulmonary and activation-regulated chemokine,Small-inducible cytokine A18,CCL18 (1-68),CCL18 (3-69),CCL18 (4-69),CCL18,AMAC1,DCCK1,MIP4,PARC,SCYA18
Available
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	9849 MW
Gene Name	CCL18
Gene Full Name	C-C motif chemokine 18
Manufacturer - Specificity	Natural and recombinant human CCL18/PARC
Background	Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: A21-A89
Manufacturer - Research Category	Cancer, Chemokines, Immunology, Innate Immunity, Oncoproteins, Tumor Biomarkers
Protein Name	C-C motif chemokine 18
Protein Function	Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
Subcellular Localization	Secreted.
Description	Human CCL18/PARC ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CCL18 in cell culture supernatants and serum.
Tissue Specificity	Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
Sample Type	cell culture supernatants and serum.
Sequence Similarities	Belongs to the intercrine beta (chemokine CC) family.
Sample Data	Concentration (pg/ml) \| 0 \| 7.8 \| 15.6 \| 31.3 \| 62.5 \| 125 \| 250 \| 500 O.D. \| 0.006 \| 0.045 \| 0.075 \| 0.186 \| 0.377 \| 0.754 \| 1.505 \| 2.145
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 49 \| 199 \| 346 \| 53 \| 218 \| 362 Standard deviation \| 1.62 \| 7.36 \| 14.88 \| 3.66 \| 15.7 \| 27.87 CV (%) \| 3.3% \| 3.7% \| 4.3% \| 6.9% \| 7.2% \| 7.7%
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ0686-CA \| Rabbit anti- human CCL18 polyclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0686-DA \| Biotinylated goat anti- human CCL18 polyclonal antibody (Detection Antibody) \| 500 µ l, 2.5 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK0686-ST \| Lyophilized recombinant human CCL18 standard \| 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Alias	C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, AMAC-1, CC chemokine PARC, Dendritic cell chemokine 1, DC-CK1, Macrophage inflammatory protein 4, MIP-4, Pulmonary and activation-regulated chemokine, Small-inducible cytokine A18, CCL18 (1-68), CCL18 (3-69), CCL18 (4-69), CCL18, AMAC1, DCCK1, MIP4, PARC, SCYA18
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EZ0686-datasheet.pdf

BOS-EZ0686-safety-datasheet.pdf