Mouse CCL3/MIP1 alpha PicoKine ELISA Kit

Manufacturer Boster
Category Products / ELISA
Type Elisa-Kit
Specific against Mouse
Applications ELISA
Amount 96 Wells
ArtNr BOS-EK0449
Eclass 6.1 32160605
Eclass 9.0 32160605
Available
Gene name
Ccl3
Assay range
7.8pg/ml-500pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of mouse CCL3 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Uniprot
P10855
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A24-A92
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Anticoagulant
heparin, EDTA or citrate
Assay procedure step1
500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml
Background
Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
Research category
|immunology|innate immunity|macrophage / inflamm.| immunology|chemokines|beta chemokines (cc)| kits/ lysates/ other|elisa kits|blood cell antigens elisa kits|immune system diseases|antiviral signaling|hiv-related
Synonyms
C-C motif chemokine 3; Heparin-binding chemotaxis protein; L2G25B; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; SIS-alpha; Small-inducible cytokine A3; TY-5; Ccl3; Mip1a, Scya3;
Gene full name
C-C motif chemokine 3
MW
10345 MW
Protein function
Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.
Subcellular localization
Secreted.
Tissue specificity
Expressed in lung, spleen, and pancreas.
Protein name
C-C motif chemokine 3
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|102|234|421|86|255|437
Standard Deviation|3.88|9.6|22.3|4.82|17 31
CV(%)|3.8|4.1|5.3|5.6|6.7|7.1
Sample data
Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500
O.D.|0.106|0.134|0.166|0.226|0.345|0.529|1.028|1.704
Tmb Incubation time
20-25min
Principle
monoclonal antibody from rat|polyclonal antibody from goat
Amount: 96 Wells
Available: In stock
Listprice: €302.73
Discount: -10%
Price: €272.46
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Salesmanager: Arne Pelz

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Safety datasheet

BOS-EK0449.pdf

Instruction sheet

BOS-EK0449.pdf