Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine®

PicoKine® ELISA Kits

11927 MW

C-X-C motif chemokine 13

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

C-X-C motif chemokine 13

Secreted.

Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles).

Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000
O.D. | 0.028 | 0.039 | 0.059 | 0.090 | 0.191 | 0.469 | 1.058 | 1.927

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 31 | 34 | 32 | 29 | 31 | 1.8 | 5.8%
Sample 2 | 182 | 204 | 199 | 194 | 194 | 8.16 | 4.2%
Sample 3 | 491 | 507 | 522 | 441 | 490 | 30.47 | 6.2%
*number of samples for each test n=16.

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.