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Mouse TREM-1 PicoKine ELISA Kit

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Manufacturer Boster
Category
Type Elisa-Kit
Specific against Mouse
Applications ELISA
Amount 96 Wells
Item no. BOS-EK0845
eClass 6.1 32160605
eClass 9.0 32160605
Available
Gene name
Trem1
Assay range
31.2pg/ml-2000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of mouse TREM-1 in cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.
Uniprot
Q9JKE2
Immunogen
Expression system for standard: NSO; Immunogen sequence: A21-S202
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.
Anticoagulant
heparin or EDTA
Assay procedure step1
2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml
Background
Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.
Research category
|immunology|innate immunity|macrophage / inflamm.
Synonyms
Triggering receptor expressed on myeloid cells 1; TREM-1; CD354; Trem1;
Gene full name
Triggering receptor expressed on myeloid cells 1
MW
25409 MW
Protein function
Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity). .
Subcellular localization
Membrane ; Single-pass type I membrane protein .
Protein name
Triggering receptor expressed on myeloid cells 1
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|252|559|924|314|675|1104
Standard deviation|14.93|39.13|42.5|24.5|48.6|60.72
CV(%)|5.9|7|4.6|7.8|7.2|5.5
Sample data
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000
O.D.|0.062|0.109|0.182|0.292|0.611|1.107|1.755|2.234
Tmb Incubation time
25-30min
Principle
monoclonal antibody from rat|polyclonal antibody from goat

Note: The presented information and documents (Manual, Product Datasheet and Safety Datasheet) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

Amount: 96 Wells
Available: In stock
available

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