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Mouse CD163 ELISA Kit PicoKine®

Mouse CD163 ELISA Kit PicoKine®

Item no.

BOS-EK1146

Manufacturer

Boster

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Mouse (Murine, Mus musculus)

Sensitivity

<20 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Scavenger receptor cysteine-rich type 1 protein M130;CD163;Soluble CD163;sCD163;Cd163;M130;

Available

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

120921 MW

Manufacturer - Gene Name

CD163

Gene Full Name

Scavenger receptor cysteine-rich type 1 protein M130

Sample Type

cell culture supernatants, serum and plasma (heparin).

Sample Data

Concentration (ng/ml)

Manufacturer - Specificity

Natural and recombinant mouse CD163

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	50 ng/tube
Anticoagulant	heparin
Background	CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
Immunogen	Expression system for standard: NS0; Immunogen sequence: V39-T1045
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Atherosclerosis, Cardiovascular, Cell Type Markers, Immunology, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation
Protein Name	Scavenger receptor cysteine-rich type 1 protein M130
Protein Function	Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium- dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity).
Subcellular Localization	Soluble CD163: Secreted.
Description	Mouse CD163 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and mesenteric lymph nodes.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK1146.pdf

BOS-EK1146-datasheet.pdf

BOS-EK1146-safety-datasheet.pdf