Comparison

Human Ki67 ELISA Kit PicoKine® European Partner

Item no. BOS-EK1759
Manufacturer Boster
Amount 96 wells/kit, with removable strips.
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity <1 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; MKI67
Available
Specificity Natural and recombinant human Ki67
Manufacturer - Category
PicoKine® ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Manufacturer - Gene Name
MKI67
Gene Full Name
marker of proliferation Ki-67
Sample Type
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Sample Data
Concentration (pg/ml)
Manufacturer - Specificity
Natural and recombinant human Ki67
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay PrecisionInter-Assay Precision
Sample
Reproducibility
LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1
Capture antibody and Detection antibody
A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Kit Components
Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
Standard
1 ng/tube
Anticoagulant
heparin or EDTA
Background
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. This gene is mapped to 10q26.2. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. Antigen KI-67 is a nuclear protein that is associated with and may be necessary
for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N3120-I3256
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Manufacturer - Research Category
Tumor Biomarkers
Protein Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome su
Subcellular Localization
Nucleus , nucleolus, Chromosome
Description
Human Ki67 ELISA Kit PicoKine® (96 Tests). Quantitate Human MKI67 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity
Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level). Present at highest level in G2 phase and during mitosis (at protein level). In interphase,

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 wells/kit, with removable strips.
Available: In stock
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