Comparison

Rat VE-Cadherin Cdh5 ELISA Kit PicoKine® European Partner

Item no. BOS-EK1916
Manufacturer Boster
Amount 96 wells/kit, with removable strips.
Category
Type Elisa-Kit
Applications ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity <50 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Cadherin 5; Cdh5
Available
Specificity Natural and recombinant rat VE-Cadherin/CD144
Manufacturer - Category
PicoKine® ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Manufacturer - Gene Name
Cdh5
Gene Full Name
cadherin 5
Sample Type
cell culture supernatants, serum and plasma (heparin, EDTA).
Sample Data
Concentration (pg/ml)
Manufacturer - Specificity
Natural and recombinant rat VE-Cadherin/CD144
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay PrecisionInter-Assay Precision
Sample
Reproducibility
LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1
Capture antibody and Detection antibody
A polyclonal antibody from goat|a biotinylated detection polyclonal antibody from goat
Kit Components
Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
Standard
20 ng/tube
Anticoagulant
heparin or EDTA
Background
Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
Immunogen
Expression system for standard: NS0; Immunogen sequence: D46 - Q599
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Manufacturer - Research Category
Atherosclerosis, Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Vascular Inflammation, Vasculature
Protein Function
Cadherins are calcium-dependent cell adhesion proteins.
Subcellular Localization
Cell membrane, Single-pass type I membrane protein
Description
Rat VE-Cadherin Cdh5 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cdh5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 wells/kit, with removable strips.
Available: In stock
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