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Mouse CLEC7A ELISA Kit PicoKine®

Mouse CLEC7A ELISA Kit PicoKine®

Item no.	BOS-EK1923
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Neuroscience Neural Lineage Markers ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<50 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	C-type lectin domain family 7 member A,Beta-glucan receptor,C-type lectin superfamily member 12,Dendritic cell-associated C-type lectin 1,DC-associated C-type lectin 1,Dectin-1,CD369,Clec7a,Bgr,Clecsf12,Dectin1
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	Clec7a
Gene Full Name	C-type lectin domain family 7, member a
Manufacturer - Specificity	Natural and recombinant mouse CLEC7A
Background	C-type lectin domain family 7 member A or Dectin-1 is a protein that in humans is encoded by the CLEC7A gene. This gene is mapped to 6; 6 F3. CLEC7A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with a partial immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor for a variety of β-1, 3-linked and β-1, 6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Expression is found on myeloid dendritic cells, monocytes, macrophages and B cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Immunogen	Expression system for standard: NS0; Immunogen sequence: G71-L244
Protein Name	C-type lectin domain family 7 member A
Protein Function	Lectin that functions as pattern receptor specific for beta-1, 3-linked and beta-1, 6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
Subcellular Localization	Cell membrane. Single-pass type II membrane protein.
Description	Mouse CLEC7A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Clec7a in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Detected in spleen (at protein level). Highly expressed in dendritic cells, spleen and thymus. Detected in epidermal Langerhans cells. Detected in macrophages, liver and lung.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 \| 4000 O.D. \| 0.066 \| 0.107 \| 0.159 \| 0.255 \| 0.449 \| 0.795 \| 1.426 \| 2.116
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 66 \| 330 \| 1181 \| 87 \| 372 \| 1349 Standard deviation \| 2.77 \| 14.19 \| 56.69 \| 4.26 \| 23.81 \| 91.73 CV (%) \| 4.2% \| 4.3% \| 4.8% \| 4.9% \| 6.4% \| 6.8%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 66 \| 62 \| 69 \| 69 \| 66 \| 2.87 \| 4.3% Sample 2 \| 330 \| 359 \| 343 \| 341 \| 343 \| 10.35 \| 3.0% Sample 3 \| 1181 \| 1329 \| 1177 \| 1283 \| 1242 \| 65.56 \| 5.2% *number of samples for each test n=16.
Capture antibody and Detection antibody	a monoclonal antibody from rat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK1923.pdf

BOS-EK1923-datasheet.pdf

BOS-EK1923-safety-datasheet.pdf