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Human CD55 ELISA Kit PicoKine®

Human CD55 ELISA Kit PicoKine®

Item no.	BOS-EK1948
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Neuroscience Neuroinflammation ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<50 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Complement decay-accelerating factor,CD55,CR,DAF
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	CD55
Gene Full Name	CD55 molecule (Cromer blood group)
Manufacturer - Specificity	Natural and recombinant human CD55
Background	Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Immunogen	Expression system for standard: NS0; Immunogen sequence: D35-S353
Manufacturer - Research Category	Cell Type Markers, Immunology
Protein Function	This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.
Subcellular Localization	Cell membrane, Single-pass type I membrane protein, GPI-anchor, Secreted
Description	Human CD55 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD55 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 O.D. \| 0.134 \| 0.176 \| 0.203 \| 0.259 \| 0.377 \| 0.667 \| 1.200 \| 1.989
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 541 \| 2056 \| 8927 \| 588 \| 2124 \| 9804 Standard deviation \| 23.26 \| 133.64 \| 419.57 \| 36.46 \| 104.08 \| 696.08 CV (%) \| 4.3% \| 6.5% \| 4.7% \| 6.2% \| 4.9% \| 7.1%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 541 \| 588 \| 498 \| 497 \| 531 \| 37.39 \| 7.0% Sample 2 \| 2056 \| 2136 \| 2100 \| 2036 \| 2082 \| 38.83 \| 1.8% Sample 3 \| 8927 \| 8943 \| 9804 \| 10335 \| 9502 \| 597.53 \| 6.2% *number of samples for each test n=16.
Capture antibody and Detection antibody	A monoclonal antibody from mouse\|a biotinylated detection polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK1948.pdf

BOS-EK1948-datasheet.pdf

BOS-EK1948-safety-datasheet.pdf