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Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

Item no.	BOS-EK2107
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology By Cell Type Carcinoma Hematology Lymphoma Myeloma Sarcoma Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<50 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Plasma kallikrein,Fletcher factor,Kininogenin,Plasma prekallikrein,PKK,Plasma kallikrein heavy chain,Plasma kallikrein light chain,KLKB1,KLK3
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	Klkb1
Manufacturer - Specificity	Natural and recombinant mouse KLKB1
Background	Plasma kallikrein?is a?protein?that in humans is encoded by the?KLKB1?gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen	Expression system for standard: NS0; Immunogen sequence: G20-A638
Protein Function	The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Subcellular Localization	Secreted
Description	Mouse KLKB1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Klkb1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.023 \| 0.151 \| 0.288 \| 0.492 \| 0.811 \| 1.242 \| 1.611 \| 2.006
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 247 \| 1236 \| 4806 \| 295 \| 1245 \| 4921 Standard deviation \| 11.12 \| 70.45 \| 221.08 \| 20.95 \| 85.91 \| 314.94 CV (%) \| 4.5% \| 5.7% \| 4.6% \| 7.1% \| 6.9% \| 6.4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 252 \| 275 \| 235 \| 272 \| 258 \| 16.19 \| 6.2% Sample 2 \| 1856 \| 1776 \| 1691 \| 1826 \| 1787 \| 62.48 \| 3.4% Sample 3 \| 4296 \| 4210 \| 3832 \| 4617 \| 4238 \| 279.56 \| 6.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	a polyclonal antibody from goat\|a biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK2107.pdf

BOS-EK2107-datasheet.pdf

BOS-EK2107-safety-datasheet.pdf