8-Isoprostane ELISA kit

All components are stored below -20°C and should not be re-frozen and thawed more than necessary.

The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF2), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity. 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers. 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations. Plasma from healthy volunteers contains modest amounts of 8-isoprostane (40-100 pg/ml) that increase with the age of the test subject.5 Normal human urinary levels range from 10-50 ng/mmol creatinine, which is an order of magnitude higher than many enzymatically derived eicosanoids.

This competitive ELISA kit is for determination of 8-isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Each kit is for triplicate analyses of up to 24 samples and contains one 96 well plate, 8-isoprostane standard, 8-isoprostane-conjugated horseradish peroxidase (HRP), and buffers for sample and HRP dilutions, and plate washing.