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p27 Kip1 Colorimetric Cell-Based ELISA Kit

p27 Kip1 Colorimetric Cell-Based ELISA Kit

Item no.	BOS-EKC1419
Manufacturer	Boster
Amount	1 kit, containing one 96-well plate and all necessary reagents
Category	Oncology By Cell Type Carcinoma Hematology Lymphoma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	>5000 cells
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Cyclin-dependent kinase inhibitor 1B,Cyclin-dependent kinase inhibitor p27,p27Kip1,CDKN1B,KIP1
Available
Manufacturer - Category	Cell-Based ELISA Kits
Storage Conditions	Store at 4°C for up to 6 months.
Molecular Weight	22073 MW
Gene Name	CDKN1B
Gene Full Name	Cyclin-dependent kinase inhibitor 1B
Protein Function	Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A- CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Subcellular Localization	Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).
Description	The p27 Kip1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p27 Kip1 protein expression profile in cells. The kit can be used for measuring the relative amounts of p27 Kip1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p27 Kip1.
Tissue Specificity	Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EKC1419-datasheet.pdf

BOS-EKC1419-safety-datasheet.pdf