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SMC1 Colorimetric Cell-Based ELISA Kit

SMC1 Colorimetric Cell-Based ELISA Kit

Item no.	BOS-EKC1531
Manufacturer	Boster
Amount	1 kit, containing one 96-well plate and all necessary reagents
Category	Oncology By Cell Type Carcinoma Hematology Leukemia ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Sensitivity	>5000 cells
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Structural maintenance of chromosomes protein 1A,SMC protein 1A,SMC-1-alpha,SMC-1A,Sb1.8,SMC1A,DXS423E,KIAA0178,SB1.8,SMC1,SMC1L1
Available
Manufacturer - Category	Cell-Based ELISA Kits
Storage Conditions	Store at 4°C for up to 6 months.
Molecular Weight	143233 MW
Gene Name	SMC1A
Gene Full Name	Structural maintenance of chromosomes protein 1A
Protein Function	Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Subcellular Localization	Nucleus. Chromosome. Chromosome, centromere, kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere- kinetochore complex at the kinetochore region during mitosis.
Description	The SMC1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SMC1 protein expression profile in cells. The kit can be used for measuring the relative amounts of SMC1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SMC1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EKC1531-datasheet.pdf

BOS-EKC1531-safety-datasheet.pdf