Histone H2A.X (Phospho-Ser139) Colorimetric Cell-Based ELISA Kit

Store at 4°C for up to 6 months.

H2AFX

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

The Histone H2A.X (Phospho-Ser139) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Histone H2A.X (Phospho-Ser139) protein expression profile in cells. The kit can be used for measuring the relative amounts of Histone H2A.X (Phospho-Ser139) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Histone H2A.X (Phospho-Ser139).