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Human P-Selectin/CD62P EZ-Set™ ELISA Kit (DIY Antibody Pairs) European Partner

Item no. BOS-EZ0505
Manufacturer Boster
Amount 5 plates/kit
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity <5 pg/ml
Sequence Belongs to the selectin/LECAM family.
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias P-selectin,CD62 antigen-like family member P,Granule membrane protein 140,GMP-140,Leukocyte-endothelial cell adhesion molecule 3,LECAM3,Platelet activation dependent granule-external membrane protein,PADGEM,CD62P,SELP,GMRP,GRMP
Available
Manufacturer - Category
EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight
90834 MW
Gene Name
SELP
Gene Full Name
P-selectin
Manufacturer - Specificity
Natural and recombinant human sP-Selectin
Background
P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
Immunogen
Expression system for standard: NS0; Immunogen sequence: W42-A771
Manufacturer - Research Category
Adaptive Immunity, Angiogenesis, Atherosclerosis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Hematopoietic Progenitors, Immunology, Myeloid, Platelets, Stem Cells, Vascular Inflammation
Protein Name
P-selectin
Protein Function
Ca (2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
Subcellular Localization
Membrane; Single-pass type I membrane protein.
Description
Human P-Selectin/CD62P ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human SELP in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Tissue Specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Sample Type
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sequence Similarities
Belongs to the selectin/LECAM family.
Sample Data
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000
O.D. | 0.033 | 0.139 | 0.273 | 0.474 | 0.728 | 1.264 | 1.714 | 2.099
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material
EZ0505-CA | Mouse anti-human P-Selectin/CD62P monoclonal antibody (Capture Antibody) | 500 µ l, 4 µ g/mL
(recommended dilution 1:100)
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0505-RB-DA | Biotinylated goat anti-human P-Selectin/CD62P polyclonal antibody (Detection Antibody) | 500 µ l (recommended dilution 1:100)
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µ l (recommended dilution 1:100)
EZ0505-RB-ST | Lyophilized recombinant human P-Selectin/CD62P standard | 10 ng/tubex3
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
Material Required But Not Provided
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 plates/kit
Available: In stock
available

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