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Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Item no.	BOS-EZ0929
Manufacturer	Boster
Amount	5 plates/kit
Category	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	C9orf26,DVS27,DVS27 related protein,IL-1F11,IL-33,IL1F11,IL33,IL33_HUMAN,Interleukin-1 family member 11,Interleukin-33,NF-HEV,NFEHEV,Nuclear factor from high endothelial venules,RP11-575C20.2
Available
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	IL33
Gene Full Name	interleukin 33
Manufacturer - Specificity	Natural and recombinant human IL-33
Background	Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: S112-T270
Manufacturer - Research Category	Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins
Protein Function	Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an alarmin, that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
Subcellular Localization	Secreted, Nucleus, Chromosome, secretory vesicle
Description	Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL33 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
Tissue Specificity	Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.029 \| 0.057 \| 0.101 \| 0.189 \| 0.335 \| 0.632 \| 1.152 \| 2.098
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 30 \| 260 \| 484 \| 28 \| 282 \| 511 Standard deviation \| 1.74 \| 16.38 \| 37.75 \| 1.76 \| 22.27 \| 45.99 CV (%) \| 5.8% \| 6.3% \| 7.8% \| 6.3% \| 7.9% \| 9%
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ0929-CA \| Rabbit anti- human IL33 polyclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0929-DA \| Biotinylated goat anti- human IL33 polyclonal antibody (Detection Antibody) \| 500 µ l, 3.5 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK0929-ST \| Lyophilized recombinant human IL33 standard \| 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin or EDTA
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Alias	C9orf26, DVS27, DVS27 related protein, IL-1F11, IL-33, IL1F11, IL33, IL33_HUMAN, Interleukin-1 family member 11, Interleukin-33, NF-HEV, NFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EZ0929-datasheet.pdf

BOS-EZ0929-safety-datasheet.pdf