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Mouse Angiopoietin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Mouse Angiopoietin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Item no.	BOS-EZ1296
Manufacturer	Boster
Amount	5 plates/kit
Category	Oncology By Cell Type ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Angiopoietin-1,ANG-1,Angpt1,Agpt
Available
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	ANGPT1
Gene Full Name	angiopoietin 1
Manufacturer - Specificity	Natural and recombinant mouse Angiopoietin-1
Background	Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
Immunogen	Expression system for standard: NS0; Immunogen sequence: H16-F497
Manufacturer - Research Category	Angiogenesis, Cardiovascular, Growth Factors
Protein Function	Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme (By similarity).
Description	Mouse Angiopoietin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse ANGPT1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.043 \| 0.082 \| 0.165 \| 0.271 \| 0.405 \| 0.713 \| 1.219 \| 1.849
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 351 \| 1479 \| 5183 \| 351 \| 1592 \| 4701 Standard deviation \| 27.02 \| 97.61 \| 207.32 \| 30.53 \| 117.8 \| 230.34 CV (%) \| 7.7% \| 6.6% \| 4% \| 8.7% \| 7.4% \| 4.9%
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ1296-CA \| Rabbit anti- mouse Angpt1 polyclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1296-DA \| Biotinylated goat anti- mouse Angpt1 polyclonal antibody (Detection Antibody) \| 500 µ l, 2.3 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK1296-ST \| Lyophilized recombinant mouse Angpt1 standard \| 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin or EDTA
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-EZ1296-datasheet.pdf

BOS-EZ1296-safety-datasheet.pdf