Comparison

Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit European Partner

Item no. BOS-FEK0407
Manufacturer Boster
Amount 96 wells/kit, with removable strips.
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity <2 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Interleukin-5;IL-5;B-cell differentiation factor I;Eosinophil differentiation factor;T-cell replacing factor;TRF;IL5;
Available
Manufacturer - Category
Fast ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight
15238 MW
Manufacturer - Gene Name
IL5
Gene Full Name
Interleukin-5
Sample Type
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Sample Data
Concentration (pg/ml)
Manufacturer - Specificity
Natural and recombinant human IL-5
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay PrecisionInter-Assay Precision
Sample
Reproducibility
LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1
Capture antibody and Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
Kit Components
Description|Quantity
96-well plate|1
lyophilized Standard|
Biotinylated detection antibody (50x)|100 ul
Avidin-Biotin-Peroxidase Complex (30x)|400 µl
Sample diluent|30 ml
Antibody diluent|12 ml
Avidin-Biotin-Peroxidase Diluent|12 ml
Wash Buffer (25x)|20 ml
Color Developing Reagent (TMB)|10 ml
Stop Solution|10 ml
Plate Sealers|4
Standard
10 ng/tube
Anticoagulant
heparin or EDTA
Background
Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
Immunogen
Expression system for standard: NS0; Immunogen sequence: I20-S134
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Protein Name
Interleukin-5
Protein Function
Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.
Subcellular Localization
Secreted.
Description
Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sequence Similarities
Belongs to the IL-5 family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 wells/kit, with removable strips.
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?