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Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit

Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit

Item no.	BOS-FEK0407
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<2 pg/ml
Sequence	Belongs to the IL-5 family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-5,IL-5,B-cell differentiation factor I,Eosinophil differentiation factor,T-cell replacing factor,TRF,IL5
Available
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	15238 MW
Gene Name	IL5
Gene Full Name	Interleukin-5
Manufacturer - Specificity	Natural and recombinant human IL-5
Background	Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
Immunogen	Expression system for standard: NS0; Immunogen sequence: I20-S134
Protein Name	Interleukin-5
Protein Function	Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.
Subcellular Localization	Secreted.
Description	Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Sequence Similarities	Belongs to the IL-5 family.
Sample Data	Concentration (pg/ml) \| 0 \| 7.8 \| 15.6 \| 31.3 \| 62.5 \| 125 \| 250 \| 500 O.D. \| 0.005 \| 0.078 \| 0.150 \| 0.269 \| 0.501 \| 0.862 \| 1.545 \| 2.201
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 9 \| 73 \| 238 \| 8 \| 70 \| 234 Standard deviation \| 0.7 \| 4.67 \| 16.42 \| 0.74 \| 4.55 \| 20.12 CV (%) \| 7.8% \| 6.4% \| 6.9% \| 9.3% \| 6.5% \| 8.6%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 9 \| 10 \| 10 \| 9 \| 9 \| 0.5 \| 5.5% Sample 2 \| 73 \| 73 \| 74 \| 62 \| 70 \| 4.92 \| 7% Sample 3 \| 238 \| 246 \| 219 \| 235 \| 234 \| 9.81 \| 4.1% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-FEK0407-datasheet.pdf

BOS-FEK0407-safety-datasheet.pdf