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Human P-Selectin / CD62P PicoKine® Quick ELISA Kit

Human P-Selectin / CD62P PicoKine® Quick ELISA Kit

Item no.	BOS-FEK0505
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Neuroscience Neural Signaling Neural Adhesion Molecules ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<5 pg/ml
Sequence	Belongs to the selectin/LECAM family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	P-selectin,CD62 antigen-like family member P,Granule membrane protein 140,GMP-140,Leukocyte-endothelial cell adhesion molecule 3,LECAM3,Platelet activation dependent granule-external membrane protein,PADGEM,CD62P,SELP,GMRP,GRMP
Available
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Calculated Molecular weight	90834 MW
Gene Name	SELP
Gene Full Name	P-selectin
Manufacturer - Specificity	Natural and recombinant Human SELP
Background	P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
Immunogen	Expression system for standard: NS0; Immunogen sequence: W42-A771
Protein Name	P-selectin
Protein Function	Ca (2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
Subcellular Localization	Membrane; Single-pass type I membrane protein.
Description	Human P-Selectin / CD62P PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SELP in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sequence Similarities	Belongs to the selectin/LECAM family.
Sample Data	Concentration (pg/ml) \| 0 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 O.D. \| 0.030 \| 0.338 \| 0.629 \| 1.146 \| 1.876 \| 2.351 \| 2.300 \| 2.348
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 322 \| 1820 \| 5020 \| 331 \| 1849 \| 5389 Standard deviation \| 15.63 \| 121.94 \| 256.02 \| 21.18 \| 144.22 \| 301.78 CV (%) \| 4.7% \| 6.7% \| 5.6% \| 6.4% \| 7.8% \| 5.6%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 322 \| 316 \| 323 \| 357 \| 329 \| 16.1 \| 4.8% Sample 2 \| 1820 \| 1756 \| 2051 \| 1775 \| 1850 \| 118.06 \| 6.3% Sample 3 \| 5020 \| 5825 \| 5171 \| 5716 \| 5433 \| 343.86 \| 6.3% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Anti-tag Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 Antibody A\|1\|3ml Antibody B\|1\|3ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	10 ng/tube
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-FEK0505-datasheet.pdf

BOS-FEK0505-safety-datasheet.pdf