Human Angiopoietin-1 / ANG1 PicoKine® Quick ELISA Kit

Description|Quantity
96-well plate|1
lyophilized Standard|
Biotinylated detection antibody (50x)|100 ul
Avidin-Biotin-Peroxidase Complex (30x)|400 µl
Sample diluent|30 ml
Antibody diluent|12 ml
Avidin-Biotin-Peroxidase Diluent|12 ml
Wash Buffer (25x)|20 ml
Color Developing Reagent (TMB)|10 ml
Stop Solution|10 ml
Plate Sealers|4

heparin or EDTA

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Angiogenesis

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Human Angiopoietin-1 / ANG1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human ANGPT1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.