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Rat EGF ELISA Kit PicoKine®

Rat EGF ELISA Kit PicoKine®

Item no.

BOS-EK0954

Manufacturer

Boster

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Rat (Rattus norvegicus)

Sensitivity

<1 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Pro-epidermal growth factor;EGF;Epidermal growth factor;Egf;

Available

Manufacturer - Category

PicoKine® ELISA Kits

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular weight

124126 MW

Manufacturer - Gene Name

EGF

Gene Full Name

Pro-epidermal growth factor

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant rat EGF

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Background	Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: N974-R1026
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction
Protein Name	Pro-epidermal growth factor
Protein Function	EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
Subcellular Localization	Membrane; Single-pass type I membrane protein.
Description	Rat EGF ELISA Kit PicoKine® (96 Tests). Quantitate Rat Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sequence Similarities	Contains 9 EGF-like domains.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

BOS-EK0954.pdf

BOS-EK0954-datasheet.pdf

BOS-EK0954-safety-datasheet.pdf