Comparison

Rat IFN gamma ELISA Kit EZ-Set™ (DIY Antibody Pairs) European Partner

Item no. BOS-EZ0374
Manufacturer Boster
Amount 5 plates/kit
Category
Type Elisa-Kit
Applications ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity <6 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Interferon gamma,IFN-gamma,Ifng
Available
Manufacturer - Category
EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name
Ifng
Gene Full Name
interferon, gamma
Manufacturer - Specificity
Natural and recombinant rat IFN gamma
Background
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E23-C156
Manufacturer - Research Category
Cancer, Cytokines, Immunology, Innate Immunity, Interferons, Signaling Pathways, Stem Cells, TGF Beta, Tumor Immunology
Protein Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subcellular Localization
Secreted.
Description
Rat IFN gamma ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Ifng in cell culture supernatants and serum.
Tissue Specificity
Released primarily from activated T lymphocytes.
Sample Type
cell culture supernatants and serum.
Sample Data
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000
O.D. | 0.087 | 0.128 | 0.163 | 0.241 | 0.484 | 0.748 | 1.356 | 2.093
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 112 | 763 | 1479 | 105 | 746 | 1389
Standard deviation | 5.71 | 24.42 | 42.89 | 9.03 | 55.95 | 79.17
CV (%) | 5.1% | 3.2% | 2.9% | 8.6% | 7.5% | 5.7%
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material
EZ0374-CA | Rabbit anti- rat Ifng polyclonal antibody (Capture Antibody) | 500 µ l, 0.4 mg/mL
(recommended dilution 1:100)
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0374-DA | Biotinylated goat anti- rat Ifng polyclonal antibody (Detection Antibody) | 500 µ l, 20 µ g/mL
(recommended dilution 1:100)
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µ l (recommended dilution 1:100)
EK0374-ST | Lyophilized recombinant rat Ifng standard | 10 ng/tubex3
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.
Material Required But Not Provided
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 plates/kit
Available: In stock
available

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